Tuesday, August 16, 2016

Oncostatin M Proteins

OSM (Oncostatin M) is a secreted cytokine involved in homeostasis and most chronic illnesses. OSM, a member of the IL-6 (gp130) family of proteins, inhibits the proliferation of a number of tumor cell lines and plays a significant role in hematopoiesis, mesenchymal stem cell differentiation, liver regeneration, heart remodeling, nociception, inflammation and metabolism. Of all IL-6-type cytokines studied so far, OSM displays the broadest signaling profile. LIF and OSM proteins activate the JAK/STAT pathway, the MAPK protein kinases ERK1 and ERK2, the stress-activated protein kinases p38 and c-Jun N-terminal kinase (JNK), the phosphatidylinositol-3-kinase (PI3K) AKT pathway and protein kinase C delta (PRKCD). In addition, OSM exhibits many other effects by regulating the production of other cytokines including IL-6, G-CSF and GM-CSF in endothelial cells. OSM may be a tumor promoter that will provide a potential target for antiangiogenic therapy.

Kingfisher Biotech's current portfolio of Oncostatin products, includes bovine and canine recombinant proteins with more in the development queue.

Kingfisher Biotech, Inc. is committed to accelerating basic veterinary research and human disease research done in animal models by developing and commercializing research reagents specific for a variety of species, including bovine, canine, caprine, chicken, dolphin, equine, feline, guinea pig, hamster, human, mouse, ovine, rabbit, rat, swine, and turkey. We will continue to add species as our customer request specific reagents for their research. Currently, we offer over 1200 recombinant proteins, polyclonal antibodies, monoclonal antibodies and ELISA kits for animal health and animal model research. Our products are primarily cytokines, chemokines, interferons, and growth factors. Our product development queue is filled mainly by customer requests. If there is a product that you are looking for and can't find, please send us an email.


Thursday, August 11, 2016

Bovine CXCL10 (IP-10) ELISA VetSet, 2 Plates

The Bovine CXCL10 (IP-10) VetSet (ELISA Development Kit) is for the quantitative determination of bovine CXCL10 (IP-10) in cell culture supernatants.


Kit Contents

Bovine CXCL10 (IP-10) Coated Plate (2 plates)
Bovine CXCL10 (IP-10) Standard
Bovine CXCL10 (IP-10) Detection Antibody
Streptavidin-HRP
VS1196B-002
C-X-C motif chemokine 10 (CXCL10) also known as Interferon gamma-induced protein 10 (IP-10) or small-inducible cytokine B10 is a member of the C-X-C chemokine family. CXCL10 (IP-10) is secreted by several cell types in response to IFN-gamma. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 (IP-10) has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis. 

Tuesday, July 12, 2016

Canine IL-8 ELISA

Interleukin-8 (IL-8), also known as CXCL8, is a CXC family member chemokine produced by macrophages and other cell types such as epithelial cells. There have been 17 different CXC chemokines described in mammals, that are subdivided into two categories, those with a specific amino acid sequence (or motif) of glutamic acid-leucine-arginine (or ELR for short) immediately before the first cysteine of the CXC motif (ELR-positive), and those without an ELR motif (ELR-negative). ELR-positive CXC chemokines such as IL-8 specifically induce the migration of neutrophils, and interact with chemokine receptors CXCR1 and CXCR2.

The Canine IL-8 Do-It-Yourself ELISA from Kingfisher Biotech contains capture antibody, standard, and detection antibody for development of a Canine IL-8 ELISA. The antibodies have been determined to function in an ELISA with the standard provided. 

Component
Quantity
Catalog #
Capture Antibody
100 µg
Detection Antibody
50 µg
Standard
5 µg

Wednesday, July 6, 2016

Cynomolgus Monkey IL-6 ELISA

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is also produced from muscle, and is elevated in response to muscle contraction. It is significantly elevated with exercise, and precedes the appearance of other cytokines in the circulation. Osteoblasts secrete IL-6 to stimulate osteoclast formation. Smooth muscle cells in the tunica media of many blood vessels also produce IL-6 as a pro-inflammatory cytokine. The role of IL-6 as an anti-inflammatory cytokine is mediated through its inhibitory effects on TNF-alpha and IL-1, and activation of IL-1ra and IL-10.

The Cynomolgus Monkey IL-6 Do-It-Yourself ELISA from Kingfisher Biotech contains capture antibody, standard, and detection antibody for development of a Cynomolgus Monkey IL-6 ELISA. The antibodies have been determined to function in an ELISA with the standard provided.


Tuesday, June 28, 2016

Swine IL-1 alpha ELISA

The IL-1 family of cytokines encompasses eleven proteins that each share a similar β-barrel structure and bind to Ig-like receptors. Several of the well characterized members of the IL-1-like cytokines play key roles in the development and regulation of inflammation. IL-1α (IL-1F1), IL-1β (IL-1F2), and IL-18 (IL-1F4) are well-known inflammatory cytokines active in the initiation of the inflammatory reaction and in driving Th1 and Th17 inflammatory responses. In contrast, IL-1 receptor antagonist (IL-1ra; IL-1F3) and IL-36 receptor antagonist (IL-36ra; IL-1F5) reduce inflammation by blocking the binding of the agonist receptor ligands. IL-33 (IL-1F11) is thought to function as an 'alarmin' released following cell necrosis to alerting the immune system to tissue damage or stress. The biological properties of IL-37 (IL-1F7) are mainly those of down-regulating inflammation.

Kingfisher Biotech's Swine IL-1α (IL-1F1) Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Swine IL-1α (IL-1F1) ELISA. The antibodies have been determined to function in an ELISA with the standard provided.



Wednesday, June 22, 2016

The Role of BAFF in B cell Biology and Autoimmunity

In healthy individuals, B cell development and maturation is tightly regulated, however this delicate balance is disrupted in autoimmune disease, where B cell dysfunction can lead to a loss of selftolerance. Disrupted B-cell maturation and function and is implicated in several autoimmune diseases, including system lupus erythematosus (SLE), rheumatoid arthritis (RA), multiple sclerosis (MS), Sjögren's Syndrome (SS), and Graves' Disease, and as a result significant research has focused on understanding the mechanisms governing B-cell maturation (Pillai, Mattoo et al. 2011, Bluml, McKeever et al. 2013).  more...

Kingfisher Biotech offers a growing number of BAFF recombinant proteins.

Monday, February 29, 2016

Bovine CCL3 (MIP-1 alpha) Do-It-Yourself ELISA, ≤10 Plates

Chemokine ligand 3 (CCL3), also known as Macrophage inflammatory protein-1 alpha (MIP-1α) is a small cytokine belonging to the CC chemokine family. There are at least 27 distinct members of the C-C subgroup reported for mammals. They are characterized by two adjacent cysteines. CC chemokines induce the migration of monocytes and other cell types such as NK cells and dendritic cells. CCL3 (MIP-1α) is a chemoattractant for several different leukocytes, with varying degrees of potency (monocytes = T-cells>neutrophils>eosinophils).

The Bovine CCL3 (MIP-1α) Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Bovine CCL3 (MIP-1α) ELISA. The antibodies have been determined to function in an ELISA with the standard provided.