IL-17A Homology Across 16 Species

IL-17A is a member of the IL-17 family, which is comprised of 6 members [IL-17A, IL-17B, IL-17C, IL-17D, IL-17E (also called IL-25), and IL-17F]. IL-17 family members are involved in numerous immune regulatory functions, including inducing and mediating proinflammatory responses and allergic responses. Il-17 induces the production of many other cytokines (IL-6, G-CSF, GM-CSF, IL-1beta, TGF-beta, and TNF-alpha), chemokines, including IL-8 (CXCL8), GRO-alpha (CXCL1) and MCP-1 (CCL2) and prostaglandins from many cell types (fibroblasts, endothelial cells, epithelial cells, keratinocytes and macrophages).

Kingfisher Biotech's portfolio of IL-17 products spans 15 species and includes proteins, antibodies, and ELISAs.

IL-17A Homology Across Species

Measurement of Equine IL-17A

PBMCs were harvested by ficoll density gradient from day old whole blood collected from an apparently healthy horse. The PBMCs were suspended at 2 x 1,000,000 cells/ml in RPMI medium containing 10% serum. PBMCs were stimulated with phytohemagglutinin (PHA; 10 µg/ml), staphylococcal enterotoxin B (SEB; 5 µg/ml) or phorbol 12-myristate 13-acetate (PMA; 10 ng/ml) and inomycin (500 ng/ml). Cell-free supernatants were harvested following three days stimulation and run in the Equine IL-17A VetSet (cat# VS0364E-002). The levels of Equine IL-17A detected are as follows:

Stimulant: Recombinant Equine IL-17A
Unstimulated: <78 pg/mL
PHA: 103 pg/mL
SEB: <78 pg/mL
PMA/Ionomycin: 283 pg/mL