Kingfisher Biotech’s Mouse GM-CSF Protein Found to be 40% More Active Than Competitor’s Mouse GM-CSF Protein

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a protein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. Granulocyte macrophage–colony stimulating factor (GM-CSF) is secreted in response to inflammatory stimuli such as LPS, IL-1, and TNF-α by a variety of different cells, including endothelium, fibroblasts, muscle cells, and macrophages, and by activated T cells. GM-CSF is glycosylated in its mature form.

Kingfisher Biotech’s mouse GM-CSF protein has been shown to be a superior product for use in cell culture. It is 40% more active than another commercially available mouse GM-CSF as measured by an independent testing laboratory. The biological activity of recombinant mouse GM-CSF was measured in a cell proliferation assay using the mouse FDC-P1 cell line. The ED50 for this effect was determined to be 0.0015-0.0023 ng/mL as compared to 0.0021-0.0035 ng/mL for the competitor’s mouse GM-CSF protein.

Mouse GM-CSF protein was produced in yeast without the use of tags. Proteins produced in yeast are naturally endotoxin free, properly folded, biologically active, and may be post-translationally modified. Many scientists find that these properties make proteins produced in yeast preferable over E. coli-produced proteins.

Mouse GM-CSF is lyophilized, available in convenient sizes (5, 25, and 100 micrograms) and in-stock ready to ship. Additional information, including product specifications and ordering information can be found at http://www.kingfisherbiotech.com.