Recent Citations that used the Bovine TNF alpha VetSet

The Kingfisher Biotech Bovine TNF alpha VetSet was used in two recent articles by M. Garcia from the University of Florida in Gainsville. In the article entitled "The effect of citrus-derived oil on bovine blood neutrophil function and gene expression in vitro" (M Garcia, TH Elsasser, D Biswas, KM Moyes - Journal of Dairy Science, 2015 - Elsevier) the authors reported that TNF-α production was not altered by TCO (cold-pressed terpeneless Valencia orange oil) when compared with controls. Results show that 0.01% TCO does not impair the function of PMNL in vitro, indicating that TCO inhibits bacterial growth without negatively altering PMNL function.Further studies will be required to determine if this a potential treatment of mastitis. In the article entitled "Increasing intake of essential fatty acids from milk replacer benefits performance, immune responses, and health of preweaned Holstein calves" (M. Garcia, J.H. Shin, A. Schlaefli, L.F. Greco, F.P. Maunsell, J.E.P. Santos, C.R. Staples, W.W. Thatcher - Journal of Dairy Science, 2015 - Elsevier), the authors reported that based upon growth performance and immune functions (measurement of bovine TNF alpha and IFN gamma) that unweaned Holstein calves benefited from consuming 3-5 grams per day of linoleic acid and 0.3-0.6 grams per day of alpha-linolenic acid.