Recombinant bovine (left) and porcine (right) CXCL11 exhibited two major protein bands, one at approximately 9 kDa (predicted MW) and one larger protein band at approximately 13 kDa (molecular weight markers starting from bottom 7.8, 12, 22 kDa). The larger MW protein most likely represents posttranslationally modified CXCL11. Recombinant equine CXCL11 exhibited the same protein band pattern, which is unique to the CXCL11 proteins. Pichia pastoris is known to provide posttranslational modifications to secreted proteins.
Monday, February 1, 2010
Bovine and Swine CXCL11
Recombinant bovine (left) and porcine (right) CXCL11 exhibited two major protein bands, one at approximately 9 kDa (predicted MW) and one larger protein band at approximately 13 kDa (molecular weight markers starting from bottom 7.8, 12, 22 kDa). The larger MW protein most likely represents posttranslationally modified CXCL11. Recombinant equine CXCL11 exhibited the same protein band pattern, which is unique to the CXCL11 proteins. Pichia pastoris is known to provide posttranslational modifications to secreted proteins.
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