Recombinant bovine (left) and swine (right) IFN gamma exhibited two major protein bands, one at approximately 16.9/16.8 kDa (predicted MW) and one larger protein band at approximately 22 kDa (molecular weight markers starting from bottom 7.8, 12, 22 kDa). The larger MW protein most likely represents posttranslationally modified IFN gamma. Pichia pastoris is known to provide posttranslational modifications to secreted proteins. IFN gamma is a cytokine that is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN gamma expression is associated with a number of autoinflammatory and autoimmune diseases.
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