Measurement of Equine IL-17A

PBMCs were harvested by ficoll density gradient from day old whole blood collected from an apparently healthy horse. The PBMCs were suspended at 2 x 1,000,000 cells/ml in RPMI medium containing 10% serum. PBMCs were stimulated with phytohemagglutinin (PHA; 10 µg/ml), staphylococcal enterotoxin B (SEB; 5 µg/ml) or phorbol 12-myristate 13-acetate (PMA; 10 ng/ml) and inomycin (500 ng/ml). Cell-free supernatants were harvested following three days stimulation and run in the Equine IL-17A VetSet (cat# VS0364E-002). The levels of Equine IL-17A detected are as follows:

Stimulant: Recombinant Equine IL-17A
Unstimulated: <78 pg/mL
PHA: 103 pg/mL
SEB: <78 pg/mL
PMA/Ionomycin: 283 pg/mL