Recent Citations that used the Bovine TNF alpha VetSet

The Kingfisher Biotech Bovine TNF alpha VetSet was used in two recent articles by M. Garcia from the University of Florida in Gainsville. In the article entitled "The effect of citrus-derived oil on bovine blood neutrophil function and gene expression in vitro" (M Garcia, TH Elsasser, D Biswas, KM Moyes - Journal of Dairy Science, 2015 - Elsevier) the authors reported that TNF-α production was not altered by TCO (cold-pressed terpeneless Valencia orange oil) when compared with controls. Results show that 0.01% TCO does not impair the function of PMNL in vitro, indicating that TCO inhibits bacterial growth without negatively altering PMNL function.Further studies will be required to determine if this a potential treatment of mastitis. In the article entitled "Increasing intake of essential fatty acids from milk replacer benefits performance, immune responses, and health of preweaned Holstein calves" (M. Garcia, J.H. Shin, A. Schlaefli, L.F. Greco, F.P. Maunsell, J.E.P. Santos, C.R. Staples, W.W. Thatcher - Journal of Dairy Science, 2015 - Elsevier), the authors reported that based upon growth performance and immune functions (measurement of bovine TNF alpha and IFN gamma) that unweaned Holstein calves benefited from consuming 3-5 grams per day of linoleic acid and 0.3-0.6 grams per day of alpha-linolenic acid.

December Special - TNF alpha Protein

With the purchase of a 25 µg vial of TNF alpha protein, you will receive a second 25 µg vial of the same TNF alpha protein at no cost. Offer expires 12/31/2013. Not valid with other specials or offers. Limit one no cost vial per order, 5 no cost vials per customer during the period of the special. Note "December 2013 Special" on your order. Our current selection of TNF alpha proteins includes, bovine, canine, dolphin, equine, feline, guinea pig, human, mouse, ovine, rabbit, & swine. This offer is available world-wide.

Equine TNF alpha Bioactivity

Kingfisher Biotech's Equine TNFα (cat # RP0137E) was determined to be biological active by an independent laboratory in Spain. The principle of the assay is that Equine TNFα should stimulate an increase in production of IL-6 and iNOS in horse adult mesenchymal cells in culture. The Equine TNFα protein was reconstituted in 1 ml of PBS + 0.1% BSA (bovine serum albumin). The horse adult mesenchymal cells were cultured in 48 well plates, in the presence of TNFα (diluted 1:100) at a final concentration of 25 ng/ml. The cultures were maintained for 12 hours at 37 degrees C. RT-PCR and quantitative PCR (real time PCR) were used to analyze in the supernants of the cultures for IL-6 and the iNOS production. Compared to control cells, the cells stimulated with Equine TNFα had significantly higher levels of IL-6 and iNOS, showing that the Equine TNFα is biologically active.

TNF alpha Homology Across Species

TNF alpha is involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. It is produced mainly by activated macrophages.

TNF alpha is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, and inflammation, as well as to inhibit tumorigenesis and viral replication. Because of the broad role of TNF alpha, dysregulation has been implicated in a variety of diseases.