Kingfisher Biotech's Bovine CD40 Ligand Do-It-Yourself ELISA (Catalog # DIY1211B-003) contains capture antibody, protein standard, and detection antibody for development of a CD40 Ligand ELISA. The antibodies have been determined to function in an ELISA with the standard provided.
CD40 Ligand (CD40LG) is a transmembrane protein that is a member of the TNF superfamily. In addition to existing in a membrane bound form, CD40LG can also be released as a soluble molecule (sCD40LG), which has been shown to have biological functions.
CD40 interacts with CD40 ligand (aka CD40LG, TNFSF5 or CD154), which is predominantly expressed on activated CD4+ T-cells. CD40LG is transiently expressed on activated T-cells and plays a crucial role in B-cell function. Activated T-cells expressing CD40LG can also interact with CD40 on endothelial cells to induce production of inflammatory cytokines. The transient expression of CD40LG allows T-cells to stimulate selected CD40-bearing cells to participate in the immune response.
You can find more products for animal health and animal model research at the Kingfisher Biotech website.
Showing posts with label bovine. Show all posts
Showing posts with label bovine. Show all posts
Friday, September 23, 2016
Tuesday, August 16, 2016
Oncostatin M Proteins
OSM (Oncostatin M) is a secreted cytokine involved in homeostasis and most chronic illnesses. OSM, a member of the IL-6 (gp130) family of proteins, inhibits the proliferation of a number of tumor cell lines and plays a significant role in hematopoiesis, mesenchymal stem cell differentiation, liver regeneration, heart remodeling, nociception, inflammation and metabolism. Of all IL-6-type cytokines studied so far, OSM displays the broadest signaling profile. LIF and OSM proteins activate the JAK/STAT pathway, the MAPK protein kinases ERK1 and ERK2, the stress-activated protein kinases p38 and c-Jun N-terminal kinase (JNK), the phosphatidylinositol-3-kinase (PI3K) AKT pathway and protein kinase C delta (PRKCD). In addition, OSM exhibits many other effects by regulating the production of other cytokines including IL-6, G-CSF and GM-CSF in endothelial cells. OSM may be a tumor promoter that will provide a potential target for antiangiogenic therapy.
Kingfisher Biotech's current portfolio of Oncostatin products, includes bovine and canine recombinant proteins with more in the development queue.
Kingfisher Biotech, Inc. is committed to accelerating basic veterinary research and human disease research done in animal models by developing and commercializing research reagents specific for a variety of species, including bovine, canine, caprine, chicken, dolphin, equine, feline, guinea pig, hamster, human, mouse, ovine, rabbit, rat, swine, and turkey. We will continue to add species as our customer request specific reagents for their research. Currently, we offer over 1200 recombinant proteins, polyclonal antibodies, monoclonal antibodies and ELISA kits for animal health and animal model research. Our products are primarily cytokines, chemokines, interferons, and growth factors. Our product development queue is filled mainly by customer requests. If there is a product that you are looking for and can't find, please send us an email.
Kingfisher Biotech's current portfolio of Oncostatin products, includes bovine and canine recombinant proteins with more in the development queue.
Kingfisher Biotech, Inc. is committed to accelerating basic veterinary research and human disease research done in animal models by developing and commercializing research reagents specific for a variety of species, including bovine, canine, caprine, chicken, dolphin, equine, feline, guinea pig, hamster, human, mouse, ovine, rabbit, rat, swine, and turkey. We will continue to add species as our customer request specific reagents for their research. Currently, we offer over 1200 recombinant proteins, polyclonal antibodies, monoclonal antibodies and ELISA kits for animal health and animal model research. Our products are primarily cytokines, chemokines, interferons, and growth factors. Our product development queue is filled mainly by customer requests. If there is a product that you are looking for and can't find, please send us an email.
Thursday, August 11, 2016
Bovine CXCL10 (IP-10) ELISA VetSet, 2 Plates
The Bovine CXCL10 (IP-10) VetSet (ELISA Development Kit) is for the quantitative determination of bovine CXCL10 (IP-10) in cell culture supernatants.
Kit Contents
Bovine CXCL10 (IP-10) Coated Plate (2 plates)Bovine CXCL10 (IP-10) Standard
Bovine CXCL10 (IP-10) Detection Antibody
Streptavidin-HRP
C-X-C motif chemokine 10 (CXCL10) also known as Interferon gamma-induced protein 10 (IP-10) or small-inducible cytokine B10 is a member of the C-X-C chemokine family. CXCL10 (IP-10) is secreted by several cell types in response to IFN-gamma. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 (IP-10) has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.
Monday, February 29, 2016
Bovine CCL3 (MIP-1 alpha) Do-It-Yourself ELISA, ≤10 Plates
Chemokine ligand 3 (CCL3), also known as Macrophage inflammatory protein-1 alpha (MIP-1α) is a small cytokine belonging to the CC chemokine family. There are at least 27 distinct members of the C-C subgroup reported for mammals. They are characterized by two adjacent cysteines. CC chemokines induce the migration of monocytes and other cell types such as NK cells and dendritic cells. CCL3 (MIP-1α) is a chemoattractant for several different leukocytes, with varying degrees of potency (monocytes = T-cells>neutrophils>eosinophils).
The Bovine CCL3 (MIP-1α) Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Bovine CCL3 (MIP-1α) ELISA. The antibodies have been determined to function in an ELISA with the standard provided.
The Bovine CCL3 (MIP-1α) Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Bovine CCL3 (MIP-1α) ELISA. The antibodies have been determined to function in an ELISA with the standard provided.
Friday, February 19, 2016
VEGF-A Across Species
Kingfisher Biotech's portfolio of VEGF-A recombinant proteins includes, bovine, canine, equine, feline, mouse, ovine, rabbit , rat, swine, and zebrafish VEGF-A.
Vascular endothelial growth factor (VEGF) proteins stimulate vasculogenesis and angiogenesis. They are part of the system that restores the oxygen supply to tissues when blood circulation is inadequate. The normal function of VEGF proteins is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels. The VEGF family has six members, including VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and Placental Growth Factor (PGF). Activity of VEGF-A, as its name implies, has been studied mostly on cells of the vascular endothelium, although it does have effects on a number of other cell types (e.g., stimulation monocyte/macrophage migration, neurons, cancer cells, kidney epithelial cells). In vitro, VEGF-A has been shown to stimulate endothelial cell mitogenesis and cell migration. VEGF-A is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor (VPF).
A recent bioassay of our bovine, canine, and mouse VEGF-A based on the proliferation of HUVECs demonstrated that all three proteins are highly active on the human cell line.
Vascular endothelial growth factor (VEGF) proteins stimulate vasculogenesis and angiogenesis. They are part of the system that restores the oxygen supply to tissues when blood circulation is inadequate. The normal function of VEGF proteins is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels. The VEGF family has six members, including VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and Placental Growth Factor (PGF). Activity of VEGF-A, as its name implies, has been studied mostly on cells of the vascular endothelium, although it does have effects on a number of other cell types (e.g., stimulation monocyte/macrophage migration, neurons, cancer cells, kidney epithelial cells). In vitro, VEGF-A has been shown to stimulate endothelial cell mitogenesis and cell migration. VEGF-A is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor (VPF).
A recent bioassay of our bovine, canine, and mouse VEGF-A based on the proliferation of HUVECs demonstrated that all three proteins are highly active on the human cell line.
Thursday, February 4, 2016
Our Newest Do-It-Yourself ELISAs
Kingfisher Biotech's portfolio of Do-It-Yourself ELISAs continues to grow with the recent addition of Bovine IL-1 beta, Caprine IL-1 beta, Chicken IL-6, and Chicken IL-10 Do-It-Yourself ELISAs. Follow the links below to view product specifications and representative data.
- Bovine IL-1 beta
- Caprine IL-1 beta
- Chicken IL-6
- Chicken IL-10
- Rabbit CCL21
- Bovine IL-2
- Swine IFN-ß
- Ovine IL-21
- Swine IGF-1
- Cynomolgus Monkey TNF alpha
- Bovine LIF
- Bovine IL-8
- Feline IL-8
- Ovine IL-8
- Swine Erythropoietin (EPO)
Monday, February 1, 2016
Our Newest Proteins - 2/1/2016
Kingfisher Biotech, Inc. is committed to accelerating basic veterinary research and human disease research done in animal models by developing and commercializing research reagents specific for a variety of species. We will continue to add species as our customer request specific reagents for their research. Currently, we offer over 1500 recombinant proteins, polyclonal antibodies, monoclonal antibodies and ELISA kits for animal health and animal model research. Our products are primarily cytokines, chemokines, interferons, and growth factors. Our product development queue is filled mainly by customer requests. If there is a product that you are looking for and can't find, please send us an email.
- Canine TNFSF11 (RANKL)
- Bovine CD40 Ligand
- Human CD40 Ligand
- Ovine CCL5
- Ovine IL-10
- Canine IL-17F
- Feline BAFF
- Ovine IL-13
- Cynomolgus Monkey IL-2
- Swine CCL28
- Ferret IL-1 beta
- Bovine TNFSF11 (RANKL)
- Mouse CXCL11
- Caprine GM-CSF
- Ferret IL-4
- Canine Fas Ligand
- Swine CCL11
- Bovine CXCL13
- Human CCL11
- Mouse CXCL5
- Caprine CCL11
- Guinea Pig IL-17A
- Cynomolgus Monkey CCL11
- Caprine IFN gamma
- Ferret IL-17A
- Equine LIF
- Rat LIF
- Caprine LIF
- Cynomolgus Monkey LIF
Saturday, January 9, 2016
Bovine IL-1 beta (IL-1F2) Do-It-Yourself ELISA
The Bovine IL-1β Do-It-Yourself ELISA (Cat# DIY1111B-003) contains capture antibody, protein standard, and detection antibody for development of a Bovine IL-1β ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Components may also be purchased separately.
The IL-1 family of cytokines encompasses eleven proteins that each share a similar β-barrel structure and bind to Ig-like receptors. Several of the well characterized members of the IL-1-like cytokines play key roles in the development and regulation of inflammation. IL-1α (IL-1F1), IL-1β (IL-1F2), and IL-18 (IL-1F4) are well-known inflammatory cytokines active in the initiation of the inflammatory reaction and in driving Th1 and Th17 inflammatory responses. In contrast, IL-1 receptor antagonist (IL-1ra; IL-1F3) and IL-36 receptor antagonist (IL-36ra; IL-1F5) reduce inflammation by blocking the binding of the agonist receptor ligands. IL-33 (IL-1F11) is thought to function as an 'alarmin' released following cell necrosis to alerting the immune system to tissue damage or stress. The biological properties of IL-37 (IL-1F7) are mainly those of down-regulating inflammation.
Wednesday, July 8, 2015
New Products - July 2015
Kingfisher Biotech, Inc. is committed to accelerating basic veterinary research and human disease research done in animal models by developing and commercializing research reagents specific for a variety of species, including bovine, canine, caprine, chicken, dolphin, equine, feline, guinea pig, hamster, human, mouse, ovine, rabbit, rat, swine, and turkey. We have just started adding cynomolgus monkey and zebrafish products. Currently, we offer over 1200 recombinant proteins, polyclonal antibodies, monoclonal antibodies and ELISA kits for animal health and animal model research. Our products are primarily cytokines, chemokines, interferons, and growth factors. Our product development queue is filled mainly by customer requests. If there is a product that you are looking for and can't find, please send us an email.
Below is a list of our newest recombinant proteins with links to website with additional information on each product.
Below is a list of our newest recombinant proteins with links to website with additional information on each product.
Thursday, July 2, 2015
Bovine Leukemia Inhibitory Factor (LIF) Polyclonal Antibody Testing
Using our bovine LIF recombinant protein as the antigen, polyclonal antibodies were produced in chickens and screened for recognition of both the glycosylated and deglycosylated forms of bovine, canine, rabbit and swine LIF recombinant proteins. (Previously it was demonstrated that the Kingfisher Biotech LIF recombinant proteins were glycosylated.) The Kingfisher Biotech LIF recombinant proteins were either treated with deglycosylation enzymes or left untreated and were subsequently screened using a Western Blot. The Western Blot showed that the anti-bovine LIF polyclonal antibody recognized both the glycosylated and the deglycosylated forms of bovine (lanes 2 & 3), canine (lanes 4 & 5), rabbit (lanes 6 & 7), and swine (lanes 7 & 8) LIF recombinant proteins.
Thursday, June 4, 2015
Glycosylation of Bovine, Canine, Rabbit, and Swine Leukemia Inhibitory Factor
Kingfisher Biotech expresses recombinant proteins in yeast so they are potentially post-translationally modified. Bovine, canine, rabbit and swine Leukemia Inhibitory Factor (LIF) have calculated molecular weights of 19.8, 19.7, 19.7, and 19.7 kDa, respectively. The Kingfisher Biotech LIF recombinant proteins were treated with deglycosylation enzymes using NEB kit Protein Deglycosylation Mix (NEB Catalog No. P6039S) to determine if the protein produced in yeast is glycosylated. The results show that all four LIF recombinant proteins shifts in molecular weight when treated with the deglycosylation enzymes; the smear of higher MW material appears to be reduced to a single band migrating with the 22/28 kDa doublet standards. The bands showing the molecular weight of the deglycosylation enzymes are also visible. This data suggests Kingfisher Biotech's bovine, canine, rabbit and swine LIF recombinant protein produced in yeast are glycosylated.
MW of Standards: 7.6, 12, 22/28 doublet, 33, 48, 71, 96, 142, 195 kDa.
16% Acrylamide SDS-PAGE Gel (Expedeon)
Lane
|
Sample
|
Treatment
|
1
|
Stds-PAGEgel Two-Color SDS Protein
Ladder
| |
2
|
Bovine LIF
|
Untreated
|
3
|
Bovine LIF
|
Denatured/Deglycosylated
|
4
|
Canine LIF
|
Untreated
|
5
|
Canine LIF
|
Denatured/Deglycosylated
|
6
|
Stds-PAGEgel Two-Color SDS Protein
Ladder
| |
7
|
Rabbit LIF
|
Untreated
|
8
|
Rabbit LIF
|
Denatured/Deglycosylated
|
9
|
Swine LIF
|
Untreated
|
10
|
Swine LIF
|
Denatured/Deglycosylated
|
11
|
Stds-PAGEgel Two-Color SDS Protein
Ladder
| |
12
|
MW of Standards: 7.6, 12, 22/28 doublet, 33, 48, 71, 96, 142, 195 kDa.
16% Acrylamide SDS-PAGE Gel (Expedeon)
Thursday, May 28, 2015
New Products - May 2015
Kingfisher Biotech, Inc. is committed to accelerating basic veterinary research and human disease research done in animal models by developing and commercializing research reagents specific for a variety of species, including bovine, canine, caprine, chicken, dolphin, equine, feline, guinea pig, hamster, human, mouse, ovine, rabbit, rat, swine, and turkey. We have just started adding cynomolgus monkey and zebrafish products. Currently, we offer over 1200 recombinant proteins, polyclonal antibodies, monoclonal antibodies and ELISA kits for animal health and animal model research. Our products are primarily cytokines, chemokines, interferons, and growth factors. Our product development queue is filled mainly by customer requests. If there is a product that you are looking for and can't find, please send us an email.
Below is a list of our newest recombinant proteins and specialty products with links to website with additional information on each product.
Below is a list of our newest recombinant proteins and specialty products with links to website with additional information on each product.
- Zebrafish IL-10 (CSIF)
- Cynomolgus Monkey IL-6
- Cynomolgus TNF alpha
- Swine Leukemia Inhibitory Factor (LIF)
- Rabbit CCL3 (MIP-1 alpha)
- Canine Leukemia Inhibitory Factor (LIF)
- Rabbit Leukemia Inhibitory Factor (LIF)
- Bovine Dendritic Cell Growth Cocktail
- Bovine Annexin V Fluorescein
- Canine Annexin V Fluorescein
- Chicken Annexin V Fluorescein
- Equine Annexin V Fluorescein
- Swine Annexin V Fluorescein
Thursday, May 7, 2015
LIF Homology Across Species
Bovine, Canine Rabbit, and Swine Leukemia Inhibitory Factor Recombinant Proteins are available from Kingfisher Biotech. Leukemia inhibitory factor, or LIF, is a member of the IL-6 family. LIF affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate.
Friday, April 24, 2015
Bovine Dendritic Cell Growth Cocktail
Dendritic cells are antigen-presenting cells of the immune system. Their main function is to process antigen material and present it on the cell surface to the T cells of the immune system. They act as messengers between the innate and the adaptive immune systems.
Dendritic cells are present in those tissues that are in contact with the external environment (e.g., skin, inner lining of the nose, lungs, stomach and intestines). Once activated, dendritic cells migrate to the lymph nodes where they interact with T cells and B cells to initiate and shape the adaptive immune response. During development Dendritic cells grow branched projections, the dendrites . CD209, also know as DC-SIGN, is exclusively expressed on dentritic cells in tissues, as well as on in vitro induced monocyte-derived-DC (MoDC).
Kingfisher Biotech now offers a Bovine Dendritic Cell Growth Cocktail - Catalog Number RPC0926B-001.
Bovine Dendritic Cell Differentiation
Bovine Dendritic Cells
Thursday, April 16, 2015
New Recombinant Proteins - April 2015
Kingfisher Biotech, Inc. is committed to accelerating basic veterinary research and human disease research done in animal models by developing and commercializing research reagents specific for a variety of species, including bovine, canine, caprine, chicken, dolphin, equine, feline, guinea pig, hamster, human, mouse, ovine, rabbit, rat, swine, and turkey. We will continue to add species as our customer request specific reagents for their research. Currently, we offer over 1200 recombinant proteins, polyclonal antibodies, monoclonal antibodies and ELISA kits for animal health and animal model research. Our products are primarily cytokines, chemokines, interferons, and growth factors. Our product development queue is filled mainly by customer requests. If there is a product that you are looking for and can't find, please send us an email.
Below is a list of our newest proteins with links to website with additional information on each protein.
Below is a list of our newest proteins with links to website with additional information on each protein.
Thursday, April 9, 2015
IFN gamma Recombinant Proteins - Bovine, Canine, Chicken, Dolphin, Equine, Feline, Human, Mouse, Ovine, Rabbit, and Swine
Bovine, Canine, Chicken, Dolphin, Equine, Feline, Human, Mouse, Ovine, Rabbit, and Swine IFN gamma Recombinant Proteins
IFN-gamma, or type II interferon, is a cytokine that is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN-gamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN-gamma in the immune system stems in part from its ability to inhibit viral replication directly, but, most important, derives from its immunostimulatory and immunomodulatory effects. IFN-gamma is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.
Kingfisher Biotech offers bovine, canine, chicken, dolphin, equine, feline, human, mouse, ovine, rabbit, and swine IFN gamma recombinant proteins. Our proteins are produced in yeast, making them naturally endotoxin-free - perfect for cell culture applications.
IFN gamma Homology Across Species
Thursday, March 26, 2015
Deglycosylation of Bovine IL-6 (RP0014B)
Kingfisher Biotech expresses recombinant proteins in yeast so they are potentially post-translationally modified. Bovine IL-6 has a calculated molecular weight of 20.6 kDa. The Kingfisher Biotech Bovine IL-6 (RP0014B) recombinant protein was treated with deglycosylation enzymes using NEB kit Protein Deglycosylation Mix (NEB Catalog No. P6039S) to determine if the protein produced in yeast is glycosylated. The results show that Bovine IL-6 shifts in molecular weight when treated with the deglycosylation enzymes; the smear of higher MW material appears to be reduced to a single band migrating with the 22/28 kDa doublet standards. The bands showing the molecular weight of the deglycosylation enzymes are also visible. This data suggests Kingfisher Biotech's Bovine IL-6 recombinant protein produced in yeast is glycosylated.
MW of Standards: 7.6, 12, 22/28 doublet, 33, 48, 71, 96, 142, 195 kDa.
16% Acrylamide SDS-PAGE Gel (Expedeon)
Lane
|
Sample
|
1
|
Standards-PAGE gel Two-Color SDS
Protein Ladder
|
2
|
Bovine IL-6 KFB RP0014B-025 - untreated
|
3
|
Bovine IL-6 KFB RP0014B-025 - denatured
|
4
|
Bovine IL-6 KFB RP0014B-025 - denatured
and deglycosylated
|
5
|
Standards-PAGE gel Two-Color SDS
Protein Ladder
|
MW of Standards: 7.6, 12, 22/28 doublet, 33, 48, 71, 96, 142, 195 kDa.
16% Acrylamide SDS-PAGE Gel (Expedeon)
Thursday, March 5, 2015
Bovine Leukemia Inhibitory Factor (LIF) Recombinant Protein
Kingfisher Biotech's Bovine Leukemia Inhibitory Factor (LIF) recombinant protein can be used in cell culture, as an LIF ELISA Standard, and as a Western Blot Control. Leukemia inhibitory factor, or LIF, is a member of the IL-6 family. LIF affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. Leukemia inhibitory factor derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to LIF include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. p53 regulated LIF has been shown to facilitate implantation in the mouse model and possibly in humans. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. LIF is typically added to stem cell culture medium to reduce spontaneous differentiation.Kingfisher Biotech's Bovine Leukemia Inhibitory Factor (LIF) recombinant protein is intended for use in animal model and animal health research.
Thursday, February 26, 2015
CCL5 Recombinant Proteins - Bovine, Canine, Chicken, Equne, Guinea Pig, Human, Mouse, Swine
RANTES/CCL5 recombinant proteins can be used in cell culture, as an RANTES/CCL5 ELISA Standard, and as a Western Blot Control. CCL5, also know as RANTES (regulated and normal T cell expressed and secreted) is a member of the C-C Chemokine Family. There are at least 27 distinct members of the C-C subgroup reported for mammals. RANTES (CCL5) is chemotactic for T cells, eosinophils, and basophils, and plays an active role in recruiting leukocytes into inflammatory sites. CCL5 plays a role in many disease states, including rheumatoid arthritis, HIV, and cancer. CCL5/RANTES recombinant proteins are intended for use in animal model and animal health research.
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RP0062B-025
|
25 ug
|
||
|
RP0062B-005
|
5 ug
|
||
|
RP0454D-025
|
25 ug
|
||
|
RP0454D-005
|
5 ug
|
||
|
RP0982C-025
|
25 ug
|
||
|
RP0982C-005
|
5 ug
|
||
|
RP0070E-025
|
25 ug
|
||
|
RP0070E-005
|
5 ug
|
||
|
RP0348GP-025
|
25 ug
|
||
|
RP0348GP-005
|
5 ug
|
||
|
RP0983H-025
|
25 ug
|
||
|
RP0983H-005
|
5 ug
|
||
|
RP0984M-025
|
25 ug
|
||
|
RP0984M-005
|
5 ug
|
||
|
RP0059S-100
|
100 ug
|
||
|
RP0059S-025
|
25 ug
|
||
|
RP0059S-005
|
5 ug
|
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