Swine Fas Ligand ELISA

The Swine Fas Ligand (FasL) Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Swine Fas Ligand (FasL) ELISA. The antibodies have been determined to function in an ELISA with the standard provided.

Fas ligand (FasL), also known as CD95L and TNFSF6, is a homotrimeric type II transmembrane protein belonging to the tumor necrosis factor (TNF) superfamily. FasL plays diverse roles in the immune system, including induction of apoptotic cell death in target cells. FasL-Fas-mediated apoptosis plays fundamental roles in several immune functions. The pathway is involved in functions including regulation of T cell homeostasis and maintenance of immune privilege, apart from its well-established role in T cell-mediated cytotoxicity. Cytotoxicity via the Fas pathway is critical for prevention of pathogenic infections as well as control of tumor cell growth.

Swine Annexin V Fluorescein-Conjugated Recombinant Protein

Annexins are important in various cellular and physiological processes such as providing a membrane scaffold, which is relevant to changes in the physical shape of the cell. Annexin proteins have been shown to be involved in trafficking and organization of vesicles, exocytosis, endocytosis and also calcium ion channel formation. Annexin proteins are not exclusively intracellular protein: Annexin proteins are also found in the extracellular space and have been linked to several processes fibrinolysis, coagulation, inflammation and apoptosis. Annexin proteins are all capable of binding negatively charged phospholipids in a calcium dependent manner and contain a 70 amino acid repeat sequence called an annexin repeat.

Kingfisher Biotech’s Swine Annexin V Fluorescein-conjugated recombinant protein has been shown to be a superior product for use in flow cytometry applications.

APRIL Homology Across Species

APRIL is a member of the TNF ligand family. This protein and its receptor are both found to be important for B cell development. In vivo experiments suggest an important role for APRIL in the long-term survival of plasma cells in the bone marrow. Mice deficient in APRIL showed a reduced ability to support plasma cell survival. APRIL may be able to induce apoptosis through its interaction with other TNF receptor family proteins such as TNFRSF6/FAS and TNFRSF14/HVEM.

Kingfisher Biotech is in the process of making recombinant APRIL from several species commercially available. Watch www.kingfisherbiotech.com for more information.