Canine IL-8 ELISA

Interleukin-8 (IL-8), also known as CXCL8, is a CXC family member chemokine produced by macrophages and other cell types such as epithelial cells. There have been 17 different CXC chemokines described in mammals, that are subdivided into two categories, those with a specific amino acid sequence (or motif) of glutamic acid-leucine-arginine (or ELR for short) immediately before the first cysteine of the CXC motif (ELR-positive), and those without an ELR motif (ELR-negative). ELR-positive CXC chemokines such as IL-8 specifically induce the migration of neutrophils, and interact with chemokine receptors CXCR1 and CXCR2.

The Canine IL-8 Do-It-Yourself ELISA from Kingfisher Biotech contains capture antibody, standard, and detection antibody for development of a Canine IL-8 ELISA. The antibodies have been determined to function in an ELISA with the standard provided. 

Component	Quantity	Catalog #
Capture Antibody	100 µg	PB1164B-100
Detection Antibody	50 µg	PBB1165B-050
Standard	5 µg	RP0101D-005

Cynomolgus Monkey IL-6 ELISA

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is also produced from muscle, and is elevated in response to muscle contraction. It is significantly elevated with exercise, and precedes the appearance of other cytokines in the circulation. Osteoblasts secrete IL-6 to stimulate osteoclast formation. Smooth muscle cells in the tunica media of many blood vessels also produce IL-6 as a pro-inflammatory cytokine. The role of IL-6 as an anti-inflammatory cytokine is mediated through its inhibitory effects on TNF-alpha and IL-1, and activation of IL-1ra and IL-10.

The Cynomolgus Monkey IL-6 Do-It-Yourself ELISA from Kingfisher Biotech contains capture antibody, standard, and detection antibody for development of a Cynomolgus Monkey IL-6 ELISA. The antibodies have been determined to function in an ELISA with the standard provided.

Swine IL-1 alpha ELISA

The IL-1 family of cytokines encompasses eleven proteins that each share a similar β-barrel structure and bind to Ig-like receptors. Several of the well characterized members of the IL-1-like cytokines play key roles in the development and regulation of inflammation. IL-1α (IL-1F1), IL-1β (IL-1F2), and IL-18 (IL-1F4) are well-known inflammatory cytokines active in the initiation of the inflammatory reaction and in driving Th1 and Th17 inflammatory responses. In contrast, IL-1 receptor antagonist (IL-1ra; IL-1F3) and IL-36 receptor antagonist (IL-36ra; IL-1F5) reduce inflammation by blocking the binding of the agonist receptor ligands. IL-33 (IL-1F11) is thought to function as an 'alarmin' released following cell necrosis to alerting the immune system to tissue damage or stress. The biological properties of IL-37 (IL-1F7) are mainly those of down-regulating inflammation.

Kingfisher Biotech's Swine IL-1α (IL-1F1) Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Swine IL-1α (IL-1F1) ELISA. The antibodies have been determined to function in an ELISA with the standard provided.

The Role of BAFF in B cell Biology and Autoimmunity

In healthy individuals, B cell development and maturation is tightly regulated, however this delicate balance is disrupted in autoimmune disease, where B cell dysfunction can lead to a loss of selftolerance. Disrupted B-cell maturation and function and is implicated in several autoimmune diseases, including system lupus erythematosus (SLE), rheumatoid arthritis (RA), multiple sclerosis (MS), Sjögren's Syndrome (SS), and Graves' Disease, and as a result significant research has focused on understanding the mechanisms governing B-cell maturation (Pillai, Mattoo et al. 2011, Bluml, McKeever et al. 2013).  more...

Kingfisher Biotech offers a growing number of BAFF recombinant proteins.

Bovine CCL3 (MIP-1 alpha) Do-It-Yourself ELISA, ≤10 Plates

Chemokine ligand 3 (CCL3), also known as Macrophage inflammatory protein-1 alpha (MIP-1α) is a small cytokine belonging to the CC chemokine family. There are at least 27 distinct members of the C-C subgroup reported for mammals. They are characterized by two adjacent cysteines. CC chemokines induce the migration of monocytes and other cell types such as NK cells and dendritic cells. CCL3 (MIP-1α) is a chemoattractant for several different leukocytes, with varying degrees of potency (monocytes = T-cells>neutrophils>eosinophils).

The Bovine CCL3 (MIP-1α) Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Bovine CCL3 (MIP-1α) ELISA. The antibodies have been determined to function in an ELISA with the standard provided.

Equine Erythropoietin (EPO) Do-It-Yourself ELISA, ≤10 Plates

The Equine Erythropoietin Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Equine EPO ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined.

Erythropoietin (EPO) is an essential hormone for red blood cell production. Without it, definitive erythropoiesis does not take place. Erythropoietin has its primary effect on red blood cell progenitors and precursors by promoting their survival through protecting these cells from apoptosis. It is the primary erythropoietic factor that cooperates with various other growth factors (e.g., IL-3, IL-6, glucocorticoids, and SCF) involved in the development of erythroid lineage from multipotent progenitors. Erythropoietin has a range of actions including vasoconstriction-dependent hypertension, stimulating angiogenesis, and inducing proliferation of smooth muscle fibers.

Swine Fas Ligand ELISA

The Swine Fas Ligand (FasL) Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Swine Fas Ligand (FasL) ELISA. The antibodies have been determined to function in an ELISA with the standard provided.

Fas ligand (FasL), also known as CD95L and TNFSF6, is a homotrimeric type II transmembrane protein belonging to the tumor necrosis factor (TNF) superfamily. FasL plays diverse roles in the immune system, including induction of apoptotic cell death in target cells. FasL-Fas-mediated apoptosis plays fundamental roles in several immune functions. The pathway is involved in functions including regulation of T cell homeostasis and maintenance of immune privilege, apart from its well-established role in T cell-mediated cytotoxicity. Cytotoxicity via the Fas pathway is critical for prevention of pathogenic infections as well as control of tumor cell growth.